UNIT I KINETICS AND MECHANISM OF ENZYME ACTION 8
Classification of enzymes; quantification of enzyme activity and specific activity.
Estimation of Michaelis Menten parameters, Effect of pH and temperature on enzyme
activity, kinetics of inhibition. Modeling of rate equations for single and multiple substrate
reactions.
UNIT II IMMOBILISED ENZYME REACTIONS 9
Techniques of enzyme immobilisation-matrix entrapment, ionic and cross linking, column
packing; Analysis of mass transfer effects of kinetics of immobilised enzyme reactions;
Analysis of Film and Pore Diffusion Effects on Kinetics of immobilized enzyme reactions;
calculation of Effectiveness Factors of immobilized enzyme systems; Bioconversion
studies with immobilized enzyme packed -bed reactors.
UNIT III MASS TRANSFER EFFECTS IN IMMOBILISED ENZYME SYSTEMS
5
Analysis of film and Pore diffusion Effects on kinetics of immobilised enzyme reactions;
Formulation of dimensionless groups and calculation of Effectiveness Factors
UNIT IV APPLICATION OF ENZYMES 12
Extraction of commercially important enzymes from natural sources; Commercial
applications of enzymes in food, pharmaceutical and other industries; enzymes for
diagnostic applications. Industrial production of enzymes. Use of enzymes in analysis-
types of sensing-gadgetry and methods. Case studies on application - chiral
conversion, esterification etc.,
UNIT V ENZYME BIOSENSORS 11
Applications of enzymes in analysis; Design of enzyme electrodes and case studies on
their application as biosensors in industry, healthcare and environment.
TOTAL: 45 PERIODS
REFERENCES:
1.Blanch, H.W., Clark, D.S. Biochemical Engineering, Marcel Dekker, 1997
2.Lee, James M. Biochemical Engineering, PHI, USA.
3.Bailey J.E. & Ollis, D.F. Biochemical Engineering Fundamentals,McGrawHill, 1986
4. Wiseman, Alan. Hand book of Enzyme Biotechnology, Ellis Harwood 1995.
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